Paramagnetic beads are added to the sample, and their surfaces attract nucleic acids to bind to the beads. Using a strong magnet, the beads are held in place while removing unwanted material. After washing, the genetic material is eluted from the beads in water or a low-salt buffer. Using Dynabeads magnetic beads, many separation applications can be achieved.
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Magnetic bead isolation is now one of the most popular nucleic acid extraction methods due to its scalability and automation compatibility. MagMAX bead kits and KingFisher Sample Purification Systems are designed to work together to efficiently purify a variety of nucleic acids.
Nucleic acid isolation by organic extraction involves the addition of phenol and guanidine isothiocyanate to separate the DNA, RNA, and proteins into different organic phases. Organic extraction is a low-cost method, and with advanced reagents such as TRIzol or phenol-chloroform, are straightforward processes requiring less lab equipment.
After lysis is performed and the solvents are added, the mixture then goes through a process of centrifugation to separate the aqueous phase (containing nucleic acids) from the organic phase (containing proteins and lipids).
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Nucleic acids bind to silica (also known as glass fibers) under high-salt conditions and can be released under low-salt conditions. Silica-containing columns provide an easy way to bind, wash, and elute purified nucleic acids from multiple clarified cell lysates in parallel.
Invitrogen Purelink and GeneJET columns are designed to flow buffers through centrifugation, vacuum, or gravity. Most protocols use spin column technology to take advantage of readily available lab equipment. Spin plates provide a high-throughput format based on the same isolation principle.
The KingFisher PlasmidPro Maxi Processor leverages the same processes and chemistry as traditional spin column-based plasmid purification methods with the advantages of automation. With prefilled reagents in a single cartridge, it helps eliminate the need for setup, centrifugation, and pipetting, reducing errors in sample preparation. This fully automated system with its "press and go" design is the only one of its kind for maxi-scale plasmid DNA purification.
Thermo Fisher Scientific offers a wide range of plasmid DNA extraction products that are designed to isolate plasmid DNA at the purity and scale you need and to support column-based workflows. Choose the PureLink or GeneJet kits based on purity grade and culture volume. Use our selection tool to find the right plasmid purification kit for your research.
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